The NBTC minicourse series will be continuing in the fall. Course details, scheduling, and pricing information are below.                 

The purpose of these minicourses is to provide researchers with practical, hands-on, small group instruction in research techniques that may be new to them. All minicourses are held in Duffield Hall at Cornell University and are scheduled over three full days.

The charge for any course is $275 for academic users. Registration for class is required due to small class sizes. This class is open to any interested researchers, however, because we will be working in NBTC lab space, all those taking the course must register to become NBTC users if they do not currently have access (Information on becoming an NBTC user is here: http://www.nbtc.cornell.edu/facilities/registration/ ).

Please contact Michael Craven (mcraven@cornell.edu) to register or make inquiries.

 

 

Schedule

Sept. 10-12: Microfluidics

Oct. 15-17: Surface Modification

Nov. 19-21: Cell Culture

Dec. 10-12: Microfludics

 

Class Descriptions

 

Cell Culture Techniques: This class covers basic mammalian cell culture handling and treatment, including:

-Sterile handling of mammalian cells and cell culture materials

-Thawing, feeding, and passaging cell lines

-Identifying normal cell morphology and potential contaminants

-Safe handling of biological materials

-Cell and tissue fixing for optical microscopy

-Mounting samples for optical microscopy

-Antibody and non-antibody cell staining for optical microscopy

-Live/dead cell staining assays

-Fluorescence imaging

-Experimental Design: Materials requirements for devices using live cells, choosing appropriate culture/fixation/staining conditions, etc.

 

Microfluidics: This class covers a variety of techniques used in microfluidic device fabrication, including:

-Cleanroom lab techniques and protocols

-Fabrication of microfluidic masters using photolithography and other techniques

-Making an imprint using soft lithography

-Device setup, packaging, and imaging

-Experimental Design: Materials requirements and limitations, etc.

-Research lunch: meet with NBTC/CMM researchers who are working on microfluidic devices

 

Surface Modification: This class covers techniques for modifying and characterizing surfaces, including:

-Formation of self-assembled monolayers

-Materials preparation for surface-specific synthesis

-Critical factors in synthesizing biologically active surfaces

-Soft lithography and monolayer stamping and etching

-Instrumental analysis of monolayer chemistry, thickness, and organization using: IR spectroscopy, ellipsometry, contact angle, AFM.